hỏi về các pp định lượng viên nang Amoxicillin

[NguyenHuuTien]

Chào các bạn, minh đang cần tìm tài liệu về các pp xác định hàm lượng Amoxicilline trong viên nang Amox. mong các bạn chỉ giúp (ngoài pp quy địng trong Dược điển). Thanks!!!:nhau :nhau (

[đông tà]

Chào bạn,
PP định lượng amox thì quy định cụ thể trong các dược điển, nếu bạn muốn tìm pp khác thì chỉ có thể liên hệ nhà cung cấp (hoặc nhà sản xuất) nguyên liệu xin tiêu chuẩn cơ sở của họ. Mình không biết vì lý do gì mà bạn không dùng pp dược điển nhưng nếu bạn dùng pp ngoài dược điển sẽ tiến hành rất nhiều thí nghiệm với pp mới để thẩm định nó. Việc này rất mất nhiều thời gian.

[NguyenHuuTien]

Chào bạn,
PP định lượng amox thì quy định cụ thể trong các dược điển, nếu bạn muốn tìm pp khác thì chỉ có thể liên hệ nhà cung cấp (hoặc nhà sản xuất) nguyên liệu xin tiêu chuẩn cơ sở của họ. Mình không biết vì lý do gì mà bạn không dùng pp dược điển nhưng nếu bạn dùng pp ngoài dược điển sẽ tiến hành rất nhiều thí nghiệm với pp mới để thẩm định nó. Việc này rất mất nhiều thời gian.
Thanks bạn nhiều nha! Mình đang cần tìm tài liệu để viết luân văn nên cần tìm xem ngoài pp quy định trong dược điển người ta còn dùng các pp nào khác. Dù sao cũng cám ơn bạn nha!

[Teppi]

Thanks bạn nhiều nha! Mình đang cần tìm tài liệu để viết luân văn nên cần tìm xem ngoài pp quy định trong dược điển người ta còn dùng các pp nào khác. Dù sao cũng cám ơn bạn nha!

Hi,

Một nhắc nhở nhỏ đến bạn là khi bạn hỏi thông tin, bạn cần phải cung cấp đầy đủ lý do và yêu cầu của bạn cho mọi người. Tránh câu hỏi kiểu cầu may (http://chemvn.net/chemvn/showthread.php?t=5292) như thế.

Về câu hỏi này, có một số phương pháp định lượng Amoxicilline bên cạnh tiêu chuẩn USP 23 NF18, 100,1995.

Sự khác biệt phương pháp thường do:
- Công cụ thiết bị. VD Dùng HPLC sẽ khác cách đo từ GC, hay FITR, DRIFT và PLS,...
- Chất phụ trợ như chất tạo màu, chất tạo phức,...
- Môi trường chứa dược chất : từ bovine sẽ khác với dạng bột hay màng film...
- Giá thành và thời gian yêu cầu
...

Bạn liên hệ tới những người từ trong bài báo này để trao đồi thêm với họ. Họ rất nhiệt tình:

Determination of Amoxicillin by Flow Injection Analysis using UV-Detection, Potentiometry, and Conductometry in Pharmaceutical Preparations
Authors: Altiokka, Göksel(1); Can, Nafiz Öncü(1); Aboul-Enein, Hassan(2)
Source: Journal of Liquid Chromatography & Related Technologies, Volume 30, Numbers 9-10, January 2007 , pp. 1333-1341(9)
1: Department of Analytical Chemistry, Faculty of Pharmacy, Anadolu University, Eskisehir, Turkey
2: College of Pharmacy, University of Sharjah, Sharjah, United Arab Emirates
Abstract:
A method for direct determination of amoxicillin in pharmaceuticals using flow injection analysis (FIA) with UV detection adjusted at 228 nm, along with potentiometer and condoptometric methods are described. The best carrier solvent system consisted of an aqueous solution of methanol (10% v/v) with pH 9 pumped at a flow rate of 1 mL • min-1. The limit of detection (LOD) and quantification (LOQ) for FIA was calculated to be 3.3×10-7 M (S/N=3) and 1.0×10-7 M (S/N=10), respectively. Two pharmaceutical formulations containing amoxicillin were studied. In the analysis of the tablets, the RSD values were found to be 1.36 and 1.21 and 1.18 (for AMOKLAVIN®), 1.10 1.16 and 1.14 (for REMOXIL®) for FIA, potentiometer, and conduct metric methods, respectively. The proposed methods were applied to the pharmaceutical tablets and good results were obtained. These proposed methods are simple, fast, accurate, precise, and cost effective for the routine determination of amoxicillin in pharmaceutical preparations

Determination of amoxicillin content in powdered pharmaceutical formulations using DRIFTS and PLS
Graciele Parisotto(I); Marco Flôres Ferrão(II)*; João Carlos Furtado(II); Rolf Fredi Molz(II)
Revista Brasileira de Ciências Farmacêuticas
Print version ISSN 1516-9332
Rev. Bras. Cienc. Farm. vol.43 no.1 São Paulo Jan./Mar. 2007
I Departament of Chemistry and Physics, Universidade de Santa Cruz do Sul
II Departament of Computer Science, Universidade de Santa Cruz do Sul
Correspondence:
M. F. Ferrão
Universidade de Santa Cruz do Sul
Av. Independência, 2293
Cx. Postal 188 96815-900 - Santa Cruz do Sul - RS, Brazil
E-mail: ferrao@unisc.br

ABSTRACT
The amount of amoxicillin in pharmaceutical formulations was determined using spectra of diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS), in association with partial least squares (PLS) regression. The spectra of 24 different samples (17 for the calibration set and 7 for the validation set), which had been registered using a Nicolet Magna 550 spectrophotometer, contained 76.7-94.3% of amoxicillin in starch. The PLS models were constructed with auto-scaled or variance-scaled data, and multiplicative scatter correction (MSC). The best model presented R2=0.9936, RMSEC=0.441 and RMSEV=0.790. The analytical method proposed is non-destructive and the cost and time of analysis are very much reduced, allowing for the fast and direct determination of amoxicillin content

Study of Spectrophotometric Determination of Amoxicillin Using Sodium 1,2-Naphthoquinone-4-Sulfonate as the Chemical Derivative Chromogenic Reagent

Analytical Letters, Volume 39, Issue 4 April 2006 , pages 763 - 775

Authors: Quanmin Li a; Zhanjun Yang a
Affiliation: a College of Chemistry and Environmental Science, Henan Normal University, The Key Laboratory of Environmental Pollution Control Technology of Henan Province, Xinxiang, Henan, P.R. China

Abstract
A simple and sensitive spectrophotometric method is described for determination of amoxicillin. The method is based on a nucleophilic substitution reaction to measure the pink compound produced by the reaction of amoxicillin with sodium 1,2-naphthoquinone-4-sulfonate in pH 9.00 buffer solution. The stoichiometric ratio of the compound is 1:1, and its maximum absorption wavelength is at 468 nm, ε=3.91 103 L • mol-1 • cm-1. The Beer's law is obeyed in the range of 0.8-120 µg • mL-1 of amoxicillin. The linear regression equation is A=0.041239+0.22128 C, with 0.9994 of a linear regression correlation coefficient. The detection limit is 2.0 µg • mL-1, and average recovery is over 98.5%. This paper further optimizes the determination of amoxcillin compared to the previous methods, and the kinetic property and reaction mechanism are studied intensively. This proposed method has been successfully applied to the determination of amoxicillin in tablets and capsules. The results obtained by this method agreed well with those by the official method high pressure liquid chromatography (HPLC).

Rapid determination of amoxicillin in premixes by HPLC
Michal Doušaa, and Romana Hosmanováb,
aEcochem, a.s. Praha, Dolejškova 3, 18200 Praha, Czech Republic
bThe Central Institute for Supervising and Testing in Agriculture in Brno, The Regional Laboratory Department Plzen, Slovanská alej 20, 31760 Plzeň, Czech Republic
Journal of Pharmaceutical and Biomedical Analysis
Volume 37, Issue 2, 23 February 2005, Pages 373-377

Abstract
A rapid analytical procedure for routine identification and quantification of amoxicillin in premixes by high performance liquid chromatography was developed and tested. The ground premix samples were extracted for 10 min using 100 ml extraction mixture water–methanol (800:200, v/v). The extract was analyzed by reversed-phase on Agilent Zorbax SB-C18 column (4.6 mm × 150 mm, i.d., 5 μm particle size) with water–methanol–phosphoric acid–triethylamine (842:150:4 : 4 ) containing 10 mM hexane-1-sulfonic acid sodium salt (pH 3.5) as mobile phase. UV detection was carried out at 230 nm. The method was validated for specificity, linearity, solution stability, accuracy, precision, limit of detection, and limit of determination. The detector response for amoxicillin was linear over the selected concentration range from 2.0 to 40.0 mg ml−1 with a correlation coefficient 0.9999. The mean accuracy was 100.1% with a standard deviation of 0.6%. The limit of detection and the limit of determination are 0.1 and 0.3 mg ml−1, respectively, which corresponds to 10 and 30 mg kg−1, respectively, in real premix sample. The sample and standard solutions were stable for 4 h. The method is selective and can be used in routine analysis.


Liquid chromatographic determination of amoxicillin concentrations in bovine plasma by using a tandem solid-phase extraction method.
H J Nelis, J Vandenbranden, A De Kruif and A P De Leenheer
Laboratoria voor Medische Biochemie en voor Klinische Analyse, Rijksuniversiteit Gent, Belgium.
Antimicrob Agents Chemother. 1992 September; 36(9): 1859-1863

ABSTRACT We report a means of determining amoxicillin in bovine plasma by liquid chromatography with UV detection at 235 nm. Purification and concentration of extracts were accomplished by a tandem solid-phase extraction procedure with two reversed-phase columns. Separation of amoxicillin from interferences was improved by the incorporation of a crown ether in the solvent systems used both for the solid-phase extraction and the final high-pressure liquid chromatography. Cefadroxil was added as an internal standard. The average recovery of amoxicillin from plasma (n = 23) was 78.2 +/- 3.0%, and the within-run and between-run coefficients of variation ranged from 1.8 to 7.0%. The detection limit was estimated at 0.1 microgram/ml. This method was used to determine amoxicillin in bovine plasma after intramuscular administration of the drug.

High-performance liquid chromatographic method for potency determination of amoxicillin in commercial preparations and for stability studies.
M C Hsu and P W Hsu
Department of Health, Executive Yuan, National Laboratories of Foods and Drugs, Taipei, Taiwan, Republic of China.
Antimicrob Agents Chemother. 1992 June; 36(6): 1276-1279

ABSTRACT A reversed-phase column liquid chromatographic method was developed for the assay of amoxicillin and its preparations. The linear calibration range was 0.2 to 2.0 mg/ml (r = 0.9998), and recoveries were generally greater than 99%. The high-performance liquid chromatographic assay results were compared with those obtained from a microbiological assay of bulk drug substance and capsule, injection, and granule formulations containing amoxicillin and degraded amoxicillin. At the 99% confidence level, no significant intermethod differences were noted for the paired results. Commercial formulations were also analyzed, and the results obtained by the proposed method closely agreed with those found by the microbiological method. The results indicated that the proposed method is a suitable substitute for the microbiological method for assays and stability studies of amoxicillin preparations.

Thân,

Teppi